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Reactivity of E1 (UBA5) towards UFM1. A) Scheme depicting the reactivity of UBA5 towards UFM1‐Dha permitting the formation of either the thioether (a) or the thioester adduct (b). B) SDS‐PAGE gel of reaction of synthetic UFM1 (6) or Rho‐UFM1‐Dha (8) and recombinant UBA5.
