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Assessment of UFM1‐PA reactivity and specificity against a panel of cysteine protease subfamilies. A) Immunoblot showing time‐dependent reactivity of Flag‐Ufsp1 (murine) towards Rho‐UFM1‐PA (10), B) and time‐dependent labeling of endogenous Ufsp2 (human). C,F) DUBs Flag‐UCHL‐1 and GFP‐OTUB2 do not react with UFM1‐PA. D,E) Flag‐SENP6 and Flag‐SENP1 react with S2‐PA (=SUMO2‐PA) but are unreactive towards UFM1‐PA. For fluorescence scans and actin blots see Figure S8.
