Figure 5.

hUCMSC‐ECM‐induced migration of hBMSC by IGFBP3 signaling pathway. (a) IGFBP3 expression in the protein extract from hUCMSC‐ECM in which the seeding cells were transfected with shIgfbp3 as assessed by Western blot. (b) The expressions of CCR2 and TβRI of hBMSC induced by shIgfbp3, and shCtrl were assessed by Western blot. (c) The expressions of pSmad2/3 and Smad2/3 of hBMSC induced by shIgfbp3 and shCtrl were assessed by Western blot. (d) Transwells assay for MSC migration was induced by scaffolds; the migrated cells were stained purple with crystal violet. Scale bar: 100 µm; *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001