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DNA relaxation by supercoiling inhibitors. Plasmid pBBR1MCS‐2, isolated from Pseudomonas syringae pv. phaseolicola 1302A, shows a dose‐dependent relaxation of supercoiling by novobiocin and ciprofloxacin. pBBR1MCS‐2 was isolated following antibiotic treatment and run on a 1% agrose gel + 2.5 µg/ml chloroquine. More negatively supercoiled topoisomers migrate faster in the gel. Direction of migration in agarose gel is from top to bottom.
