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. 2018 Oct 3;110(3):444–454. doi: 10.1111/mmi.14111

Figure 5.

Figure 5

Gene expression and circular intermediate detection in a topoisomerase insertion mutant, strain Pseudomonas syringae pv. phaseolicola 1302A::56. Bacterial cells were inoculated in extracted bean cultivar Tendergreen apoplastic fluid for 5 h before gene expression of A. integrase gene xerC and C. effector gene avrPphB or B. detection of the circular intermediate was measured. Results are displayed as fold expression. D. Bacterial cells were inoculated into bean cultivar Tendergreen leaves and the plants incubated for 5 h before the apoplastic fluid was extracted and gene expression of effector gene avrPphB was measured. avrPphB and xerC expression were standardised by simultaneous qPCR analysis of acpP expression and error bars represent standard error of the mean of three experimental replicates. Letters above bars indicate significant differences at p < 0.05 assessed with Student’s t‐test. AP: extracted TG apoplastic fluid.