Table 2.
Primer name | Description | Sequence 5′–3′ |
---|---|---|
acpP | Housekeeping gene | Forward TTGGCGTCAAATCAGAAGAG |
Reverse GCTTCTTCGTCAGGGATTTC | ||
Probe ACCTGGGCGCTGACTCCCTG[Link] | ||
gyrB | gyrB gene | Forward GATGATGGAATCGGTGTCGAA |
Reverse TTGGTGAAGCACAACAGGTTCT | ||
Probe CCCTGCAGTGGAACGACAGCTTCA[Link] | ||
xerC | PPHGI‐1 encoded xerC intergrase | Forward CGACGATACGGCCTCCAA |
Reverse AAAGGTGCGGTCGACATCA | ||
Probe CCCCCTATAGCGGAGCGTCTGGAA[Link] | ||
QavrPphB | PPHGI‐1 encoded effector gene | Forward CCCATTCCTGGCAATGACA |
Reverse TTACGCCTGAAGAGGATGCA | ||
Probe TGGGCGATAAAGGG[Link] | ||
QCI | Circular intermediate | Forward CATGGGCCTTCCAGATTTTC |
Reverse CTGCGGTTTGGGATACTGAAC | ||
Probe CGTAACGCTGAGGCAGGCCCC[Link] | ||
avrPphB | PPHGI‐1 encoded effector avrPphB | Forward GCGATTGCGTGTCCTTGA |
Reverse CTGTAAGACCTGAGCCTG |
aProbes labelled with 5′ FAM and 3' TAMRA TaqMan dyes.