Figure 6.

The senescence marker p16 is limited expressed during osteoclastogenesis. Percentage of p16 positive nuclei in multinucleated, TRAP positive osteoclasts (OCL) over time (left) and representative examples (right) in normoxic (21% O₂) and hypoxic (5% O₂) culture conditions. The arrows indicate p16 positive nuclei in multinucleated cells. Note that while mononuclear cells were p16 positive, no osteoclasts were present after 1 week of culture. There were no significant differences between culture conditions