Figure 4.

SYP121^ΔC interaction with KAT1 is voltage dependent and long‐lived. Dwell time constant analysis for closed lifetimes with KAT1 before (open bars), during additions of SYP121^ΔC (grey bars), and after its washout from the bath (black bars). Shown is the largest time constant τ_C3 used as a proxy for SNARE‐channel interactions (see Figure 3). Protocols above summarize the sequence of treatments and clamp voltages used. Mean dwell time constants ±SE (n = 4) are indicated below each protocol and in Table 1. Adding SYP121^ΔC at −120 mV led to an increase in τ_C3 that did not recover on washout at −120 mV (left protocol) or 0 mV (centre protocol). SYP121^ΔC had no effect on τ_C3 when added at 0 mV (right protocol) at which the channel is normally closed [Colour figure can be viewed at http://wileyonlinelibrary.com]