Figure 4.

SDC4 influences the level of myostatin. (A) Representative western blot experiments show the levels of SDC4, promyostatin, myostatin and phospho‐pSMAD2^Ser465/467 in nontransfected C2C12 myoblasts and in cell lines stably expressing shRNA against SDC4 (shSDC4#1, shSDC4#2) or scrambled shRNA. GAPDH shows the equal loading of the samples. (B) Cell culture media of the cells shown in panel A were collected and subjected to SDS/PAGE, followed by immunoblotting with anti‐myostatin antibody. (C) Quantification of the results is reported as means ± SEM (n = 3–5 independent experiments); *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.