Figure 2.

miR‐16 inhibits the proliferation of SCC‐25 and CAL‐27 cell lines. The miR‐16 mimic/inhibitor and the corresponding negative control were transfected into SCC‐25 and CAL‐27 cell lines, respectively. The relative expressions of miR‐16 in SCC‐25 (a) and CAL‐27 (c) cells were determined at 48 hr after transfection by qRT‐PCR. Cell proliferation of SCC‐25 (b) and CAL‐27 (d) was measured at 24, 48, 72, 96, and 120 hr after transfection by the CCK‐8 assay. Data were presented as mean ± standard deviation for three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 versus negative control. miR‐16, microRNA‐16; qRT‐PCR, quantitative reverse transcription polymerase chain reaction