Figure 7.

Overexpression of BCL2L2 promotes proliferation and inhibits apoptosis in SCC‐25 cells. (a) Protein expression of BCL2L2 was measured by western blot in SCC‐25 cells after cotransfection with pcDNA3.1‐ BCL2L2 and miR‐16. (b) Cell proliferation was measured at 24, 48, 72, 96, and 120 hr after transfection by CCK‐8. (c) Annexin V/PI staining analysis was performed to investigate the apoptosis of SCC‐25 cells. (d) The histogram showed the apoptosis rate of SCC‐25 cells. Data were presented as mean ± standard deviation for three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 versus the negative control. miR‐16, microRNA‐16 [Color figure can be viewed at wileyonlinelibrary.com]