Figure 5.

CRISPR/Cas9‐mediated disruption of HBsAg decreased IL‐6 production in HCC cells. (A and B) IL‐6 protein levels in the culture medium of indicated cells were determined by ELISA. Data are presented as mean ± SD of three independent experiments. P values were calculated using a Student's t‐test. **P < 0.01, *P < 0.05. (C and D) pY‐STAT3 and total STAT3 levels of indicated HepG2‐2.15 (left) and PLC/PRF/5 (right) cell lines were analyzed by Western blotting. (E and F) pY‐STAT3 and total STAT3 levels of indicated SK‐hep1 (left) and HLF (right) cells were analyzed by Western blotting