Figure 3.

Identification of the MXD4‐NUTM1 fusion gene. A, Identification of the MXD4–NUTM1 fusion transcripts through RT‐PCR and Sanger sequencing. An electropherogram demonstrated the junction point of two genes. B, The schematics of MXD4–NUTM1 fusion transcript. The bHLH domain in MXD4 and pro‐rich domain in NUTM1 were retained in the MXD4–NUTM1 fusion transcript. C, Relative NUTM1 mRNA expression in the undifferentiated ovarian small round cell sarcoma reported in this study compared with epithelial ovarian cancers. Ovarian sarcoma denotes the present case. HGS and CCC denote high‐grade serous carcinoma and clear cell carcinoma, respectively. D, Immunohistochemical staining revealed high expression of NUTM1 with speckled nuclear pattern in the ovarian undifferentiated small round cell sarcoma reported in this study (400× magnification; scale bar, 100 μm) [Color figure can be viewed at http://wileyonlinelibrary.com]