Table 3.

Relative normalised gene expressions in stems of M. sativa. Plants were exposed to 10 mg·Cd·kg⁻¹ soil. Three reference genes were used for normalisation. Expression levels were calculated relative to the non‐exposed plants

Gene annotation	Contig_ID M. sativa	Corresponding gene ID M. truncatula	Rel. norm expression ± SE	P‐value
Beta‐like galactosidase	2998	Medtr1g018200.1	1.507 ± 0.325	0.323
Beta‐like galactosidase	1506	Medtr2g039120.1	2.272 ± 0.229	0.004
α‐L‐arabinofuranosidase/β‐xylosidase	187	Medtr2g008240.1	1.656 ± 0.157	0.007
α‐L‐arabinofuranosidase/β‐D‐xylosidase	54428	Medtr2g034720.1	2.299 ± 0.329	0.006
Alpha‐galactosidase‐like protein	33480	Medtr7g073650.1	0.966 ± 0.1	0.865
Glucan endo‐1,3‐β‐glucosidase	75119	Medtr4g076470.1	2.383 ± 0.291	0.012
Pectinesterase/pectinesterase inhibitor	93377	Medtr7g050950.1	2.854 ± 0.364	0.001
Non‐classical arabinogalactan protein 31‐like	91472		1.041 ± 0.025	0.512
Trichome birefringence‐like protein	13146	Medtr2g015720.1	0.895 ± 0.31	0.259
Xyloglucan endotransglucosylase/hydrolase family protein	102819	Medtr4g126920.1	1.065 ± 0.112	0.454
Xyloglucanase‐specific endoglucanase inhibitor protein	100138	Medtr1g072420.1	1.146 ± 0.040	0.391
Peroxidase family protein	12706	Medtr2g029850.1	1.953 ± 0.151	0.0003
Class III peroxidase	17644	Medtr4g095450.1	2.530 ± 0.057	2.45·e−6
Lignin biosynthetic peroxidase	84389	Medtr2g084020.1	5.808 ± 0.522	0.002
Peroxidase family protein	64923	Medtr6g043240.1	2.083 ± 0.235	0.005
Class III peroxidase	14966	Medtr5g074970.1	1.669 ± 0.043	5.2·e−6
Glycoside hydrolase, family 17	67100	Medtr2g034440.1	4.567 ± 0.494	9.584·e−5
Fasciclin‐like arabinogalactan protein	86958	Medtr4g059840.1	0.920 ± 0.053	0.512
Polygalacturonase non‐catalytic protein	111493	Medtr8g064530.1	1.156 ± 0.143	0.405
Dirigent protein 21‐like	4524	Medtr1g018200.1	1.716 ± 0.170	0.006

Values are mean ± SE from five biological replicates. P‐values below or equal to 0.05 indicate significant differences.