Figure 2.

 Screening data of 5′‐shortened P_GTH1 promoter variants. The geometric means of the populations’ specific eGFP fluorescence (fluorescence related to cell volume) is shown for clones expressing eGFP under control of P_GTH1 (named 965 bp) and the shortened P_GTH1 variants (two clones each which were selected in prescreenings were cultivated in triplicates) in repressing and inducing growth conditions. Wild‐type P. pastoris cells were used as negative control. Samples were taken during the repressing preculture and after 24 and 48 h induction with feed beads. eGFP: enhanced green fluorescent protein