Figure 5.

Schematic illustration of P_GTH1 promoter duplication variants. The initially cloned P_GTH1 sequence (a) is 965 bp long (amplified from position 36 to 1000 of the sequence shown here). PstI and BglII restriction sites (located at positions 509‐514 and 525‐530, indicated with scissors) were used to generate P_GTH1 variants P_GTH1 ‐D1240 and P_GTH1 ‐D1426 with duplicate fragments. Sequence fragments corresponding to a,b and a,c of sequence (a) were amplified with primers containing appropriate restriction sites and ligated into the site, thereby generating duplication variants with a length of 1240 bp (b) and 1427 bp (c) [Color figure can be viewed at wileyonlinelibrary.com]