Figure 2.

Generation of iHeps from MEFs and morphology. (a) Schematic showing the generation of iHeps. Cells were cultured in MEF medium for 2 days after transduction. From Day 2, cells were cultured in iHep medium. Cells were subcultured twice around Day 7 and Day 14, when reaching confluency. (b) Representative flow cytometry analysis of dTomato expression in untransduced MEFs (left) and iHeps (Day 16, right). (c) Morphology of untransduced MEFs cultured in MEF medium (left) and iHep medium (middle) and iHeps (right) in iHep medium for 16 days. Scale bar = 100 μm. MEFs: mouse embryonic fibroblasts [Color figure can be viewed at wileyonlinelibrary.com]