Figure 4.

Functional validation of iHeps. Relative activity of hepatocyte‐specific enzymes (a–c), Cyp3a activity (d), and albumin secretion (e) in MEFs, iHeps, and primary hepatocytes. Results were normalized with cell number. Normalized data are shown as mean ± SEM of four independent experiments for primary hepatocytes and six independent experiments for MEFs and iHeps (P17). The asterisk represents statistical significance. *p < 0.05. *p < 0.01. The hash represents nonsignificance (two‐tailed Mann–Whitney U test). AST: aspartate aminotransferase; LDH: lactate dehydrogenase; GLDH: glutamate dehydrogenase; MEFs: mouse embryonic fibroblasts; SEM: standard error of the mean