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. Author manuscript; available in PMC: 2019 Jan 16.
Published in final edited form as: Biofabrication. 2018 Jan 16;10(2):025004. doi: 10.1088/1758-5090/aa96de

Fig. 5. Heart-on-a-chip applications of UV laser micropatterning.

Fig. 5

(A) Schematic of MTFs using UV laser micropatterning. (B) UV-M engineered MTFs: (i) Stereoscope brightfield images of engineered NRVM cardiac muscular thin films in diastole and systole (ii) after 5 days in culture. Red line indicates height of MTF detected by tracking software. Blue boxes represent initial length. Scale bar is 0.5 mm. (iii) Raw contractile stress traces at 0, 1, and 2 Hz pacing frequencies for the same representative MTF. (iv) Contractile stress of UV laser micropatterned muscular thin films (n = 9–13 films, 5–6 heart chips). Bars represent the mean ± SEM for diastolic (black), systolic (white), and twitch stress (gray). (C) Beat rate of engineered MM (black) and UV-M (red) NRVM cardiac tissues in culture over a 27 day period in beats per second. (D) Contractile stress of UV-M muscular thin films after 27 days in culture (n = 2–3 films, 1 heart chip). Bars represent the mean ± SEM for diastolic (black), systolic (white), and twitch stress (gray). *P<0.05 compared to 1 Hz pacing by Student’s T-Test.