Table 1. Example of a pipetting scheme for 0.1 μM f-cAMP and increasing concentrations of SthK.

Stock solution	Volume of stock solution to be added (μl)	Final volume in cuvette (μl)	[SthK] (μM) in cuvette
400 μl volume 0.1 μM f-cAMP 50 μM SthK	0	2,000	0.00
	2	2,002	0.05
	2	2,004	0.10
	2	2,006	0.15
	2	2,008	0.20
	2	2,010	0.25
	5	2,015	0.37
	5	2,020	0.50
	5	2,025	0.62
	5	2,030	0.74
	5	2,035	0.86
	5	2,040	0.98
	10	2,050	1.22
	10	2,060	1.46
	10	2,070	1.69
	10	2,080	1.92
	10	2,090	2.15
	10	2,100	2.38
	20	2,120	2.83
	20	2,140	3.27
	20	2,160	3.70
	20	2,180	4.13
	20	2,200	4.55
	50	2,250	5.56
	50	2,300	6.52
	50	2,350	7.45