Table 2. Example of a pipetting scheme for the competition of cNMP with the complex formed between 0.3 μM f-cAMP and 0.3 μM SthK.

Stock solution	Volume of stock solution to be added (μl)	Final volume in cuvette (μl)	[cNMP] (μM) in cuvette
200 μl volume 0.3 μM f-cAMP 0.3 μM SthK 30 mM cNMP	0	2,000	0.00
	1	2,001	14.99
	1	2,002	29.97
	1	2,003	44.93
	2	2,005	74.81
	2	2,007	104.63
	3	2,010	149.25
	5	2,015	223.33
	5	2,020	297.03
	10	2,030	443.35
	10	2,040	588.24
	20	2,060	873.79
	20	2,080	1,153.85
	20	2,100	1,428.57
	20	2,120	1,698.11
	20	2,140	1,962.62
	20	2,160	2,222.22