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. 2018 Sep 17;99(5):1186–1193. doi: 10.4269/ajtmh.18-0276

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© The American Society of Tropical Medicine and Hygiene

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Figure 1. — Singleplex and multiplex qPCR efficiencies. Assay optimization to compare the efficiencies of single vs. multiplex PCR assays using gBlock gene targets for (A) Necator americanus, (B) Ancylostoma ceylanicum, and (C) Ancylostoma duodenale fragments (IDT^® Technologies) as standard curve controls. Ct = cycle threshold. This figure appears in color at www.ajtmh.org.