Table 1.
Primer and probe sets for the quantitative hookworm multiplex PCR
| Target | Primer/Probe | Sequence 5′ to 3′ | Size (bp) | Gene target | Conc. nM | Source |
|---|---|---|---|---|---|---|
| Necator americanus | NecF | CTGTTTGTCGAAC-GGTACTTGC | 101 | ITS2 | 250 | 10 |
| NecR | ATAACAGCGTGCA-CATGTTGC | 250 | ||||
| NecP | Cy5-CTGTACTACG-CATTGTATAC-IBFQ | 100 | ||||
| Ancylostoma spp. | AncF | CGGGAAGGTTGG-GAGTATC | 104 | ITS1 | 300 | This study |
| AncR | CGAACTTCGCACA-GCAATC | 300 | This study | |||
| Ancylostoma ceylanicum | AncCeyP | FAM-CCGTTCCTGG-GTGGC-IBFQ | 100 | This study | ||
| Ancylostoma duodenale | AncDuoP | HEX-TCGTTACTG-GGTGACGG-IBFQ | 100 | This study | ||
| EHV | EHVFWD | GATGACACTAGCG-ACTTCGA | 81 | gB | 40 | 15 |
| EHVREV | CAGGGCAGAAACC-ATAGACA | 40 | ||||
| EHVProbe | ROX-TTTCGCGTGC-CTCCTCCAG-IBRQ | 100 |
EHV = Equine herpesvirus; ITS = internal transcribed spacer 2.