Table 2. List of primers used for mRNA expression in AT.
| Gene | GenBank accession no. | Sequence 5`>3` |
|---|---|---|
| CNR1 | NM_001242341.1 | F:AAGCCCGCATGGACATTAGGTTAG |
| R: AGCAGAGGGCCCCAGCAGAT | ||
| CNR21 | NM_001192303.1 | F: TCTTCGCCGGCATCATCTAC |
| R: CATCCGGGCTATTCCAGACA | ||
| MGLL1 | NM_001206681.1 | F: GCAACCAGCTGCTCAACAC |
| R: AGCGTCTTGTCCTGGCTCTT | ||
| FAAH1 | NM_001099102.2 | F: TTCCTGCCAAGCAACATACCT |
| R: CACGAAATCACCTTTGAAGTTCTG | ||
| NAPEPLD1 | NM_001015680.1 | F: AGAGATCACAGCAGCGTTCCAT |
| R: ACTCCAGCTTCTTCAGGGTCATC | ||
| HSL | NM_001080220.1 | F: GAGTTTGAGCGGATCATTCA |
| R: TGAGGCCATGTTTGCTAGAG | ||
| LEP | NM_173928.2 | F: GGCTTTGGCCCTATCTGTCTTA |
| R: GAGACGGACTGCGTGTGTGA | ||
| TNFα | NM_173966.3 | F: CCATCAACAGCCCTCTGGTT |
| R: GGGCTACCGGCTTGTTACTT | ||
| FABP4 | NM_174314.2 | F: GTCATGAATGGTGTCACTGCC |
| R: GGAGTTCGATGCAAACGTCA | ||
| BRPS2 | NM_001033613.2 | F: GGAGCATCCCTGAAGGATGA |
| R: TCCCCGATAGCAACAAACG | ||
| HP | NM_001040470.2 | F: GGTTCGGAAAACCATCGCTA |
| R: CACTCGTGTCCCCTCCCTC | ||
| IL1β | NM_174093.1 | F: TCCACCTCCTCTCACAGGAAA |
| R: TACCCAAGGCCACAGGAATCT |
CNR1 = CB1 receptor, CNR2 = CB2 receptor, MGLL = monoglyceride lipase, FAAH = fatty acid amide hydrolase, NAPEPLD = N-acyl phosphatidylethanolamine-specific phospholipase D, HSL = hormone-sensitive lipase, LEP = leptin, FABP4 = fatty acid binding protein 4, TNFα = tumor necrotizing factor α, IL1β = interleukin-1β, HP = haptoglobin, BRPS2 = bovine ribosomal protein S2.
1the sequence for this gene was based on Khan et al.[28].