Fig 5. Fractionation of MAX-interacting complexes.

(A) Schematic illustration of the fractionation of ESC nuclear extracts by column chromatography. Nuclear extracts were prepared from VV3 ESCs and fractionated into fractions A-D on a heparin sepharose column. Fractions A-D were further fractionated into fractions I-IV on a Q sepharose column. (B to E) Samples immunoprecipitated using anti-MAX antibody or control IgG for fraction A-III (B), B-III (C), C-III (D), or D-III (E) were analyzed by Western blotting using anti-DNMT, anti-RING1B, or anti-MAX antibodies. Principally, the same result was obtained in two independent experiments. The un-cropped data of these images are shown in S6A–S6D Fig, respectively.