Fig 4. Structure of the Golgi apparatus in hippocampal neurons of ΔE-torsinA knock-in mice, as analyzed using the 3-image average method.

Confocal fluorescence microscopy of cultured neurons at 17–19 DIV. A: Representative confocal image of a WT neuron stained with BODIPY FL C₅-ceramide (left). Thresholded image is shown in binary format (middle). The ROI used to measure the somatic area is overlaid on the image (right). B: Representative confocal images of neurons of the WT, HET and HOM genotypes. An optical section is shown (Single), as a comparison for an image averaged from 3 sections used for analyses. C: Quantitative analysis of measured parameters. Total fluorophore (BODIPY FL C₅-ceramide) was calculated as (area) × (averaged intensity / pixel). Columns represent the means and the bars represent standard errors of the mean (SEM). There was no statistical significance (NS) in the values measured for mutants vs. WT (p>0.1; t-test; n = 20, 26, 18 neurons for WT, HET and HOM, respectively).