Fig 12. Dendritic Golgi outposts in young cultures.

A: DIC and confocal images of a cultured, BODIPY FL C₅-ceramide-stained hippocampal neuron at 11 DIV. Double-ended arrow indicates the length of longest outpost in this neuron. B: Ratios of neurons with outposts to total number of neurons. The ratios between mutant and WT neurons were not statistically significant (p>0.1; chi-squared test; total number of neurons = n = 191, 143, 98 11-DIV neurons, 35, 54, 46 17-to-19-DIV neurons, and 16, 28, 25 26-DIV neurons for WT, HET and HOM, respectively). C. Lengths of dendritic outposts. Differences in lengths of dendritic outposts between mutant and WT neurons were not statistically significant (p>0.1; t-test; n = 97, 72, 58 neurons for WT, HET and HOM, respectively). In B and C, the data obtained with ceramide staining and immunocytochemistry for GM130 were merged.