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. 2018 Nov 7;13(11):e0206123. doi: 10.1371/journal.pone.0206123

Fig 15. Degree of Golgi apparatus disruption by brefeldin A.

Fig 15

Cultured hippocampal neurons at 11 DIV, following treatment with 1 μg/ml brefeldin A or DMSO for 2 hours at 37°C and immunocytochemical staining for GM130, MAP2 and DAPI. A: Representative slide scanner images of neurons. The images are shown in an 8-bit intensity format. The same image contrast was used in each color channel (representing GM130, MAP2 or DAPI) throughout the 4 images, such that the minimum of 4 minima and the maximum of 4 maxima were 0 and 255 on an 8-bit intensity scale. The mean intensity values of GM130 in the 4 images were (from left to right): 745, 730, 261 and 281. B: Quantitation of GM130 staining intensity. There was no genotypic difference in the GM130 staining intensities when the neurons were treated with vehicle (N.S.; p = 0.822). In both genotypes, treatment with brefeldin A reduced the GM130 staining intensity in comparison to treatment with vehicle (WT, *: p = 3.04 × 10−32; HET, *: p = 3.52 × 10−18). However, there was no genotypic difference in the GM130 staining intensities after treatment with brefeldin A (N.S.; p = 0.139) (n = 139 WT and 62 HET neurons treated with DMSO only, and 85 WT and 70 HET neurons treated with brefeldin A).