Fig 1. Quantitative analysis of hemolytic activity of rNetF.

A two-fold serial dilution of rNetF was made in 96-well plates and incubated at 37°C for 1 h with RBCs (2.5% by volume) from six different species. Subsequently, the cells were pelleted and the supernatants were removed. Hemolysis was determined by measuring absorbance at 424 nm. PBS was used as the negative control for 0% hemolysis and 2% Triton X-100 as the positive control for 100% hemolysis. The values are averages of triplicate assays in three experiments, with error bars representing standard deviation.