Fig 3.

A-B. Inhibition of rNetF-induced hemolysis and cytotoxicity by PEG. A) Sheep RBCs (2.5% by volume) were incubated with EC₅₀ concentration of rNetF (1.3 μg/ml of rNetF in the presence of 30 mM PEG (molar weight as indicated) at 37°C for 1 h. Hemolysis was determined by measuring absorbance at 424 nm. B) Confluent monolayer EO cells were incubated with EC₅₀ concentration of rNetF (5 ng/ml) in the presence of 30 mM PEG (molar weight as indicated) at 37°C. Cytotoxicity was evaluated after 4 h. The values are averages of triplicate assays in three experiments, with error bars representing standard deviation. Results were considered statically significant from the control if p ≤ 0.01 (**) and p ≤ 0.0001 (****).