Table 1. Sequences 5’-3’ of Alphavirus and Flavivirus specific primers used for Nested-PCR reactions.

	PCR reaction		Nested-PCR reaction
	Name	Sequence 5’-3’	Name	Sequence 5’-3’
Alphavirus	α.nsP4.PF	TAYYTDGAYATGGTIGABGG	α.nsP4.NF	TGYAAYGTIACICARATGMG
	α.nsP4.PR	GGYTTICCIADYTTVAAIAG	α.nsP4.NR	AAICCICCRCARAARTAIGG
Flavivirus	F.NS5P-F	ACIMTBITSTGTGACATHGGIGA	Flav100F*	AAYTCIACICAIGARATGTAY
	F.NS5P-R	GTBAHIGTGTTIARGGCRTAIGT	Flav200R*	CCIARCCACATRWACCA

*Flav100F and Flav200R primers were published by Maher-Sturgess et al., 2008 [26].