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. 2018 Oct 26;14(10):e1007657. doi: 10.1371/journal.pgen.1007657

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© 2018 Tian et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — (A) PRDM9 interacts with CXXC1 and EWSR1 in spermatocytes. Co-IP with PRDM9 from 14 dpp B6 testicular extract. Staining for each protein is indicated on left. In each blot, lane 1- input; lane 2 –co-IP with non-immune IgG; lane 3 –co-IP with antibody against PRDM9. (B) EWSR1 does not interact with CXXC1 in spermatocytes. Co-IP with EWSR1 from 14 dpp B6 testicular extract. Staining for EWSR1 and CXXC1. In each blot, lane 1- input; lane 2 –co-IP with non-immune IgG; lane 3 –co-IP with anti-EWSR1 after DNase I treatment; co-IP with anti-EWSR1 without DNase I treatment. (C) CXXC1 interacts with PRDM9 but not with EWSR1 in cell culture. Myc tagged CXXC1, HA tagged EWSR1 and Flag tagged PRDM9 were transfected into HEK293 cells. Input controls (left panel), co-IP with HA antibody (middle panel), and IP with Myc antibody (right panel). In each row, staining for the indicated proteins is shown. (D) CXXC1 binds to H3K4me3 but not to H3K9me3. Co-IP with CXXC1 from 14 dpp B6 testicular extract. Top row, staining for CXXC1; middle row, staining for H3K4me3; bottom row, staining for H3K9me3.