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. Author manuscript; available in PMC: 2018 Nov 7.
Published in final edited form as: Mol Cell. 2015 Mar 19;57(6):1034–1046. doi: 10.1016/j.molcel.2015.02.015

Figure 5. C-Terminally Deleted or Modified p53 Variants can be Cross-Linked to the p21 more efficiently than the mdm2 Binding Site.

Figure 5

WT, p300-acetylated p53 (Ac), 6KQ and Δ30 p53 proteins were bound to 32P end-labeled DNA fragments containing either p21 (A) or mdm2 (B) binding sites in which the UV cross-linkable nucleotide analogue 4-thio-dTTP was present at the indicated positions (shown in bold italics within the corresponding BS sequences). Following DNA-p53 complex formation, the reaction mixtures were subjected to UV-irradiation and then separated by 8% SDS PAGE. p53-DNA cross-linked products (X-link) were visualized using a PhosphorImager. Each panel shows a portion of the gel image (at left) and the corresponding graphical analyses (at right) of the relative intensities of each cross-link. The standard deviations were calculated from three parallel experiments.