Fig. 3. Nucleosomes are reversibly destabilized in the presence of curaxins and FACT.

(A) Experimental approach. DNA-labeled nucleosomes were incubated in the presence of FACT, unlabeled competitor DNA, CBL0137 (B), or CBL0100 (C) and analyzed by native PAGE. Note that in the presence of both FACT and curaxins, nucleosomes are unstable (as reflected in release of histone-free DNA); this effect is reversed in the presence of DNA competitor that partially removes FACT from the nucleosomes (see Fig. 2).