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. 2018 Nov 7;4(11):eaau6762. doi: 10.1126/sciadv.aau6762

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Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 4 — Day 1 HSPCs were cocultured with Cy5-pmaxGFP–loaded (A and C) CMPs or (B and D) MkMPs in Eppendorf tube (Tube), FT system with centrifugation (FT + Centri.), FT supplemented with PB (8 μg/ml) (FT + PB), or the combination of PB with centrifugation in FT system (FT + PB + Centri.). Both cells and isolated nuclei were harvested for flow cytometric analysis on (A and B) Cy5⁺ nuclei percentage or (C and D) MFI of Cy5⁺ nuclei at 24 hours. Data represent averages of three biological replicates ± SEM.