Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (mus musculus) male |
TKO-Bcl2 mouse embryonic fibroblasts (MEFs) |
PMID:16338659 | ||
| Cell line (mus musculus) male |
TKO-Bcl2-p53KO MEFs | This paper | N/A | Knockout of p53 in TKO-Bcl2 MEFs, described in the materials and methods |
| Cell line (mus musculus) male |
TKO-p53RNAi MEFs | PMID:16338659 | ||
| Cell line (mus musculus) male |
TKO-p53KO MEFs | This paper | N/A | Knockout of p53 in TKO MEFs, described in the materials and methods |
| cell line (mus musculus) male |
Rb-/-Rbl2-/- mESCs | PMID: 15574596 | ||
| Cell line (mus musculus) male |
Rb-/-Rbl2-/-p53-/- mESCs | This paper | Knockout of p53 in Rb-/-Rbl2-/-, described in the materials and methods |
|
| Cell line (homo sapiens) female |
RPE-1 hTERT | ATCC | Cat# CRL-4000 | RRID:CVCL_4388 |
| Cell line (homo sapiens) female |
TKO RPE-1s | This paper | N/A | Knockout of Rb, Rbl1 and Rbl2 in RPE-1-hTERT cells, described in material and methods |
| Cell line (homo sapiens) female |
TKO-Bcl2 RPE-1s | This paper | N/A | Knockout of Rb, Rbl1, Rbl2 and overexpression of Bcl2 in RPE-1-hTERT cells, described in material and methods |
| Cell line (homo sapiens) female |
TKO-Bcl2-p53KO RPE-1s | This paper | N/A | Knockout of Rb, Rbl1, Rbl2 and TP53 and overexpression of Bcl2 in RPE-1-hTERT cells, described in material and methods |
| Cell line (homo sapiens) female |
CyclinD1 RPE-1s | This paper | N/A | Overexpression of non-degradable CyclinD1 (T286A) in RPE-1-hTERT cells, described in material and methods |
| Cell line (homo sapiens) female |
CyclinD1-Bcl2 RPE-1s | This paper | N/A | Overexpression of non-degradable CyclinD1 (T286A) and Bcl2 in RPE-1-hTERT cells, described in material and methods |
| Cell line (homo sapiens) female |
CyclinD1-Bcl2-p53KO RPE-1s |
This paper | N/A | Knockout of TP53 and overexpression of non-degradable CyclinD1 (T286A) and Bcl2 in RPE-1-hTERT cells, described in material and methods |
| Antibody | Brdu (recognizing CldU) |
Bioconnect | Cat# OBT0030G Clone BU1/75 |
RRID: AB_609567 (1/500) |
| Antibody | BrdU (recognizing IdU) |
Becton Dickinson | Cat# 347580 Clone B44 | RRID: AB_10015219 (1/750) |
| Antibody | mouse p53 | Abnova Corporation | Cat# MAB9657 Clone IMX25 | RRID: AB_10756365
(1/1000) |
| Antibody | mouse/ human p21 |
Santa Cruz | Cat# sc-397 C19 | RRID: AB_632126 (1/500) |
| Antibody | p27 | BD Transduction Laboratory | Cat# 554069 | RRID: AB_395225 (1/2000) |
| Antibody | CDK4 | Santa Cruz | Cat# sc-260 C22 | RRID: AB_631219 (1/2000) |
| Antibody | Actin | Santa Cruz | Cat# sc-1616 | RRID:AB_630836 (1/1000) |
| Antibody | pChk1 Ser 317 | Bethyl | Cat# A304-673A | RRID: AB_2620868 (1/1000) |
| Antibody | Chk1 | Santa Cruz | Cat# sc-8408 G4 | RRID: AB_627257 (1/1000) |
| Antibody | Rb1 | Santa Cruz | Cat# sc-50 C15 | RRID: AB_632339
(1/500) |
| Antibody | Rbl1 | Santa Cruz | Cat# sc-318 C18 | RRID: AB_2175428 (1/1000) |
| Antibody | Rbl2 | Lab Vision | Cat# OP117 Clone AB1 | RRID: AB_145257
(1/1000) |
| Antibody | Cyclin D1 | Santa Cruz | Cat# sc-753 H296 | RRID: AB_2070433 (1/1000) |
| Antibody | human p53 | BD Bioscience | Cat# 554293 Clone DO-1 |
RRID: AB_395348 (1/500) |
| Antibody | Bcl2 | Santa Cruz | Cat# sc-509 | RRID: AB_626733 (1/1000) |
| Chemical compound, drug | 2'-deoxyadenosine monohydrate (dA) |
Jena Bioscience | N-DN-1001 | |
| Chemical compound, drug | 2'-deoxycytidine monohydrochloride (dC) |
Jena Bioscience | N-DN-6352 | |
| Chemical compound, drug | 2'-deoxyguanosine monohydrate (dG) |
Jena Bioscience | N-DN-1003 | |
| Chemical compound, drug | 2'-deoxythymidine (dT) | Jena Bioscience | N-DN-6354 | |
| Chemical compound, drug | Cell Player 96-well kinetic caspase-3/7 reagent |
Essen Bioscience | Cat# 4440 | |
| Chemical compound, drug | CldU | Sigma | Cat# C6891-100mg | |
| Chemical compound, drug | IdU | Sigma | Cat# I7125-5g | |
| Chemical compound, drug | BrdU | Sigma | Cat# B5002-1G | |
| Chemical compound, drug | Hydroxyurea (HU) | Sigma | Cat# H8627 | |
| Chemical compound, drug | Propidium Iodide (PI) |
Thermo fisher scientific | Cat# P3566 | |
| Chemical compound, drug | Puromycin | Sigma | Cat# P7255 | |
| Chemical compound, drug | Penincillin/ Streptomycin |
Gibco/Life technologies | Cat# 15140122 | |
| Chemical compound, drug | Lipofectamine 2000 | Invitrogen | Cat# 11668030 | |
| Chemical compound, drug | Polybrene | Sigma | Cat# H9268 | |
| Chemical compound, drug | Fluor-gel with TES buffer |
Electron Microscopy Science | Cat# 17985–30 | |
| Software, algorithm | GraphPad Prism 7 |
GraphPad Software | https://www.graphpad.com/scientific-software/prism/ | |
| Software, algorithm | Adobe Photoshop CS6 |
Adobe | https://www.adobe.com/products/photoshop.html | |
| Software, algorithm | Adobe Illustrator CS6 | Adobe | https://www.adobe.com/products/illustrator.html | |
| Software, algorithm | Image Studio Lite Ver. 4.0 |
LI-COR Biosciences | https://www.licor.com/bio/products/software/image_studio_lite/ | |
| Software, algorithm | ImageJ | https://imagej.nih.gov/ij/download.html | ||
| Software, algorithm | FlowJo software version 7.6.1 |
FlowJo, LCC | https://www.flowjo.com/solutions/flowjo/downloads | |
| Software, algorithm | Casplab | Casplab | http://casplab.com/download |