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. 2018 Nov 1;198(9):1140–1150. doi: 10.1164/rccm.201712-2513OC

Figure 3.

Figure 3.

Lung natural killer cells (NKs) are more cytotoxic against epithelial cells in a murine model of cigarette smoke (CS) exposure. C57BL/6 mice were exposed to air or CS for 8 weeks, and then lung tissue was collected for the isolation of NKs (CD49b+) and CD326+ epithelial cells. Epithelial cells from air-exposed mice were cultured either alone or with NKs from air-exposed or CS-exposed mice. Likewise, epithelial cells from CS-exposed mice were cultured either alone or with NKs from air-exposed or CS-exposed mice. After 4 hours, cells were collected and stained for CD45, annexin-V, and 7-aminoactinomycin D for flow cytometry. Epithelial cells were identified as CD45− with high side scatter. The percent cytotoxicity is shown for four separate experiments (five mice per group per experiment). Box plots show the first and third quartiles plus the median, and the whiskers represent the minimum and maximum values. Repeated measures one-way ANOVA with Tukey’s multiple comparison test was used to determine significance. **P < 0.01.