Fig. 5. AMPK mediates the inhibitory effect of KMS99220 on LPS-induced iNOS expression. (A, C) BV2 cells were pretreated with Ara A (A) or Compound C (C) for 1 h, and then treated with 10 µM KMS99220 and/or 0.2 µg/ml LPS for 6 h. RT-PCR was performed against iNOS using GAPDH as a loading control. Values obtained from densitometry were normalized against GAPDH and expressed as fold of LPS-treated control. (B) BV2 cells were pretreated with Ara A for 1 h, and then treated with 10 µM KMS99220 and/or 0.2 µg/ml LPS for 24 h. Nitrite levels were assessed by the Griess method. The data are expressed as % of untreated control±SEM; ^**p<0.01.