Fig. 3. AC affected hippocampus-related animal behavior. (A left) In the ORM training, both groups exhibited equal preference levels for the two test objects. (A middle) Upon testing, vehicle-treated mice preferred the novel object, but AC-treated mice were impaired in terms of novel object recognition. (A right) The discrimination ratio was markedly lower in AC-treated mice than vehicle-treated mice, indicating that the anti-cancer treatment impaired novel object preference. (B, C) AC-treated mice were immobile for significantly more time than were vehicle-treated control mice during the TST (B) and FST (C), which evaluate depression-like behavior. These results indicate that AC treatment induced depression-like behavior. Data are expressed as means±SEs. ^**p<0.01, ^***p<0.001 vs. vehicle-treated controls.