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Impact of α-synuclein expression prior to and post differentiation with retinoic acid (RA). A) Immunofluorescence analysis to determine α-synuclein effect on differentiation markers. Pre α-Syn (+) cells were first induced with Dox for 3 days and then differentiated for 7 days. Post α-Syn (+) cells were first differentiated for 7 days and then induced with dox for 3 days. B) MAP2 fluorescence intensity per cells. Measurement from 40 cells from four different fields. ****p ≤ 0.0001.
