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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Metabolism. 2018 Sep 5;89:39–47. doi: 10.1016/j.metabol.2018.08.006

Figure 2. TET2 is necessary and sufficient to facilitate Rosi-mediated insulin sensitization in vitro.

Figure 2.

(A) Mature 3T3-L1 adipocytes at day 8 were lentivirally transduced with single-guide RNA specific for Tet2 (sgTet2) or an empty plasmid vector (sgCont). Two days after transduction, cells were treated with TNF (4 ng/ml), Rosi (100 nM), or both for 2 additional days and assessed for insulin-stimulated glucose uptake (3H-2-DG assay, n = 6, P < 0.05). (B, C) Basal and insulin-stimulated glucose uptake in mature 3T3-L1 adipocytes (B) and immortalized human adipocytes (C) transduced with lentivirus expressing the catalytic domain of wild-type (Tet2-CD) or mutated (Tet2-HxD) Tet2 or GFP (n = 6, p < 0.05, Student’s t-test, mean ± s.e.m.).