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. 2018 Nov 1;8:467. doi: 10.3389/fonc.2018.00467

Figure 1.

Figure 1

Results of droplet digital PCR (ddPCR) on cell-free DNA of ten patients, grouped by WHO2010 grade (G), functionality of the tumor (NF, non-functional; INS, insulinoma) and presence of metastasis (M0, no metastasis; M1, metastasis present). The graph shows the number of positive droplets per 1 mL of plasma, for both mutant (black) and wild-type (WT; gray) target. For mutant-positive patients, the variant allele fractions (VAFs) are indicated. The selected ddPCR targets are, from left to right, chr3:g.98251584T>A (GPR15), chr2:g.46603856C>T (EPAS1), chr21:g.39754856C>A (ERG), chr20:g.46288182C>T (SULF2), chr5:169477296C>T (DOCK2), chr2:g.111416130T>G (BUB1), chr2:g.204073466C>G (NBEAL1), chr16:g.15854467G>A (MYH11), chr11:g.64575561C>A (MEN1), chr6:g.33288573G>A (DAXX) (in GRCh37/hg19). For case 7, results obtained with the same ddPCR assay are shown for the perioperative plasma sample (T1) and the follow-up plasma sample (T1+23 months).