. Author manuscript; available in PMC: 2019 Nov 6.

Published in final edited form as: Structure. 2018 Sep 6;26(11):1499–1512.e5. doi: 10.1016/j.str.2018.07.012

Table 3.

Steady-State Kinetic Parameters for TbDHSc:DHSp and Mutants

	NAD⁺		Spermidine
	k_cat (s⁻¹)	K_M (μM)	k_cat(s⁻¹)	K_M (μM)
WT	0.15 ± 0.0045	1.3 ± 0.18	0.18 ±0.010	9.8 ± 1.2
E166A-DHSc:DHSp	0.16 ± 0.00057	1.2 ±0.20	0.12 ± 0.010	11 ± 1.5
DHSc:DHSp-H266A	0.16± 0.010	98 ± 26	0.081 ± 0.01	12 ± 1.8

Experiments were conducted at 5 nM DHS and 15 μM TbeIF5A. Substrate concentrations were: (1) NAD⁺ K_M, NAD⁺ (1.3–1,000 μM) and [¹⁴C] spermidine (78 μM); (2) spermidine K_M, NAD⁺ (1 mM) and [¹⁴C]spermidine (0.12–78 μM). Error represents the SE of the fit for triplicate data.