Figure 4.

Ex-vivo expansion of Vδ2⁺ T cells from haploHSCT recipients after stimulation with pamidronate-pretreated DCs. Peripheral blood lymphocytes (PBLs) from transplant recipients were directly treated with pamidronate or cocultured with monocyte-derived and pamidronate-pretreated autologous or allogeneic (refers to third-party healthy subjects) DCs, which are referred to as auto-DCs^pami+ or allo-DCs^pami+ respectively. (A) Representative images of flow cytometric analyses of Vδ2⁺ T cell expansion in different groups after 14 days of culture. (B) The proportions of Vδ2⁺ T cells in each of 6 recipients after different treatments for 7 and 14 days. (C) The average fold changes in Vδ2⁺ T cells expansion in different groups (n = 6). (D) The average proportions of Ki67⁺Vδ2 cells in different groups (n = 3). (E) The proportions of Vδ2⁺ T cell fractions with various differentiation statuses in different groups (n = 6). (F) The proportions of Vδ2⁺ T cells after coculture with auto-DCs^pami+ or allo-DCs^pami+, with or without NKG2D blocking antibody (n = 3). P-values are shown on the graphs.