Figure 10.

Analysis of expression of M1-associated markers (NOS2, IL-1β, IL-6, TNF, Cox-2) in LPS-activated macrophage cell line with overexpression of Egr2. Macrophage cell line RAW264.7 was transfected with empty (control) pMIG expression vector plasmid [V(C)] or vector with Egr2 [V(Egr2)] for 24 h and after which the cells were used as unstimulated [V(C) and V(Egr2)] or activated with LPS (100 ng/ml) for another 24 h-time period [V(C)+LPS and V(Egr2)+LPS] as in Figure 7. The cells were washed, mRNA was isolated and the expressions of NOS2 (A), IL-1β (B), IL-6 (C), TNF (D), Cox-2 (E) were analyzed by real-time PCR as described in Materials and Methods. In (A–E), mean ± S.E. of six separate culture plate wells is shown (^****p < 0.0001; ^*****p < 0.00001; ^******p < 0.000001; ^*******p < 0.0000001).