Table 2.

Effect of Egr2 knockdown on expression of macrophage activation markers MHC class II and CD86 during inflammatory conditions^a.

	M0 (Unstimulated)		M2 (IL-4)		M1 (IFNγ)		Thioglycollate-induced inflammation
	Control siRNA	Egr2 siRNA	Control siRNA	Egr2 siRNA	Control siRNA	Egr2 siRNA	Control siRNA	Egr2 siRNA
MHC class II	257 ± 16	259 ± 27	282 ± 9	311 ± 7	611 ± 35	208 ± 39b	1836 ± 228	905 ± 86c
CD86	67 ± 7	74 ± 5	83 ± 8	99 ± 6	282 ± 8	170 ± 8c	173 ± 3	136 ± 10d

^aBMDMs were grown from bone marrow of B6 or DsRed transgenic mice in the presence of M-CSF for 5 days and transfected with Control siRNA or Egr2 siRNA. The cells were analyzed in vitro or injected i.p. into the group of 4–5 mice with thioglycollate-induced inflammation. For in vitro analysis, the cells were incubated in media alone (unstimulated) or in the presence of IL-4 (50 ng/ml) or IFNγ (100 ng/ml) as described in Materials and Methods. After 24 of incubation in vitro or on day 4 after induction of thioglycollate-elicited inflammation, the cells were stained for MHC class II, CD86, and F4/80. The F4/80⁺ gated (in vitro) or F4/80⁺DsRed⁺ (ex-vivo isolated) gated cells were analyzed for expression of MHC class II and CD86 by 3–4-color flow cytometry and mean fluorescence intensity (MFI) levels for expression of MHC class II and CD86 were measured. Mean ± S.E. of three separate experiments or 4–5 individual animals is shown.

^bP < 0.001 when compared to control siRNA.

^cP < 0.01 when compared to control siRNA.

^dP < 0.01 when compared to control siRNA.