Figure 4.

Inflammatory Responses after stimulation with different S. Typhi strains. Cells from the 3-D model were untreated (none) or exposed to CVD 908, CVD 909, CVD 910, CVD 915, Ty2, or Ty21a S. Typhi strains. After 4 h, tissues were collected and used to measure the gene expression by qRT-PCR. Data shown are unsupervised clustergram displaying hierarchical clustering of the genes related to the inflammatory response signaling as a heat map as a heat map. Geometric mean values of two independent experiments with two replicates. Results are shown as x-fold regulation relative to the control group. Genes detected by the Antimicrobial Responses RT² Profiler PCR Array.