Figure 7.

Differential expression of mucus and antimicrobial peptide signaling after stimulation with different S. Typhi strains. Cells from the 3-D model were untreated (none) or exposed to CVD 908, CVD 909, CVD 910, CVD 915, Ty2, or Ty21a S. Typhi strains. After 4 h, tissues were collected and used to measure the gene expression by qRT-PCR. (A) Data shown are unsupervised clustergram displaying hierarchical clustering of the genes related to the antimicrobial signaling as a heat map. Geometric mean values of two independent experiments with two replicates. Results are shown as x-fold regulation relative to the control group. Genes detected by the Antibacterial Response RT² Profiler PCR Array. (B) Mucus (MUC2) gene expression. Bar graphs extend from the 25 to 75th percentiles, and the line in the middle represents the median of the pooled data. The whiskers delineate the smallest to the largest value. The data represent six individual experiments with one or two replicates. Horizontal lines represent significant differences (p < 0.05) between the indicated culture conditions.