Table 4.
Aim 2 Variables
| Variable | Method of Measurement | Operational Definition | Level of Measurement |
|---|---|---|---|
| High Wound Bioburden | Swab specimen processed using 16 S rRNA sequencing | Microbial load (number of bacterial cells) Microbial Diversity Shannon index Faith’s index Relative Abundance of Staph Genre Anaerobic Genre |
Continuous Continuous Continuous Continuous Continuous Continuous |
| High Inflammatory mediators | Swab specimens processed to extract cDNA using qPCR | Cycle threshold (Ct) values | Continuous |
| Low Wound pH | pH Paper (6.5-13.0 pH with 0.5 incremental readings) | pH value | Continuous |