Figure 1.

Naive brain dimensions, water content, and viscoelasticity. a, Brain dimensions of naive juvenile and adult CD1 mice were assessed on Nissl stained coronal brain slices Cortical mantle thickness (T) is the length of the cortical layer. The width (W) and height (H) were determined as the distance from medial-lateral and dorsal-ventral, respectively. b, Juvenile mice showed a significant increase in the percentage of overall water content of the brain (**p = 0.0046) as well as between each hemisphere (right, **p = 0.0020; left **p = 0.0071) compared with adult (c). d, No significant differences were seen between the width (in millimeters) of adult and juvenile mice whether combined or analyzed by bregma levels −1.5, −1.8, and −2.1. e, f, No significant difference in combined height or by bregma levels −1.5, −1.8, and −2.1 was observed. g, h, Cortical mantle thickness (CMT) was significantly increased in juvenile compared with adult (**p = 0.0010.). i, CMT by bregma levels −1.8 and −2.1 was also increased at −1.8 and −2.1 compared with adult (**p = 0.0017 and ***p = 0.0008, respectively). j, No significant difference was observed between the elasticity (p = 0.50 sham; p = 0.50 injured) or viscosity (p = 0.27 sham; p = 0.61 injured) (k) of juvenile and adult sham-injured or 4 d injured brains.